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Promega pgl3 basic empty control plasmid
Pgl3 Basic Empty Control Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+basic+empty+control+plasmid/pgl3+basic/10__1080_slash_2162402x__2018__1500107-189-19-24
Average 90 stars, based on 1 article reviews
pgl3 basic empty control plasmid - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Control:

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or post-differentiated 3T3-L1 cells grown in 24-well plates were co-transfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3- Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO- Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or postdifferentiated 3T3-L1 cells grown in 24-well plates were cotransfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3-Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO-Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Plasmid Preparation:

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or post-differentiated 3T3-L1 cells grown in 24-well plates were co-transfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3- Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO- Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or postdifferentiated 3T3-L1 cells grown in 24-well plates were cotransfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3-Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO-Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Construct:

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or post-differentiated 3T3-L1 cells grown in 24-well plates were co-transfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3- Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO- Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or postdifferentiated 3T3-L1 cells grown in 24-well plates were cotransfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3-Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO-Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Luciferase:

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or post-differentiated 3T3-L1 cells grown in 24-well plates were co-transfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3- Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO- Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or postdifferentiated 3T3-L1 cells grown in 24-well plates were cotransfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3-Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO-Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Transfection:

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or post-differentiated 3T3-L1 cells grown in 24-well plates were co-transfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3- Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO- Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.

Article Title: Adipose PD-L1 Modulates PD-1/PD-L1 Checkpoint Blockade Immunotherapy Efficacy in Breast Cancer
Article Snippet: Transient transfection was performed using Lipofectamine 2000 (Invitrogen). .. For promoter study, B16, pre- or postdifferentiated 3T3-L1 cells grown in 24-well plates were cotransfected with 0.1 μg of pGL3 basic empty control plasmid (Promega, Cat: #E1751) or pGL3-Cd274 promoter construct and 0.02 μg of the Renilla luciferase construct (Promega, Cat: #E6911, for normalization of transfection efficiency). .. For 3’UTR study, pmirGLO (Promega, Cat: #E1330) or pmirGLO-Cd274 3’UTR vector were transfected into pre- or post-differentiated 10T1/2 cells.



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